SubtiBank SubtiBank
yxiM [2019-06-24 10:41:48]
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yxiM [2019-06-24 10:41:48]

similar to rhamnogalacturonan acetylesterase
locus
BSU39120
pI
6.62
mw
41.68 kDa
protein length
382 aa Sequence Blast
gene length
1149 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    4,017,508 → 4,018,656

    The protein

    Protein family

  • 'GDSL' lipolytic enzyme family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|578B79EB64B804FCAACA73AB22458481371A815C|YesY], [protein|585CA3154C0405697C4707E5B1D9359963999C54|YesT]
  • Structure

  • [PDB|2O14]
  • Expression and Regulation

    Operons

    genes
    [gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]-[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]
    description
    [Pubmed|10746760]

    regulation

  • expression declines in the stationary phase [Pubmed|23175651]
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    Biological materials

    Mutant

  • MGNA-B719 (yxiM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1718 NBRP B. subtilis, Japan]
  • GP1116 (spc), available in [SW|Stülke] lab
  • BKE39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT, downstream forward: _UP4_TAATACATCATTTTTCGACA
  • BKK39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT, downstream forward: _UP4_TAATACATCATTTTTCGACA